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Proceedings Paper

Measurement of mammalian erythrocyte indices from light scattering with scanning flow cytometer
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Paper Abstract

The technique of scanning flow cytometery (SFC) was adopted for measurement of erythrocyte indices: volume, hemoglobin concentration and surface area. The method has been verified on two types of mammalian red cells: human and murine. In order to access distribution of values, precision and stability of inverse algorithm of reconstitution of size and refractive index of microsphere from light scattering angular dependency (indicatrix) have been increased performing analysis of Fourier spectrum of indicatrix. Volume - hemoglobin concentration (V-HbC) map was obtained following well-known procedure of isovolumetric sphering, presented by Technicon Instruments. Additionally new method for measurement of paired distribution of erythrocyte surface area (S) and hemoglobin content (Hb) was implemented via registration of spherical stage in the course of colloid-osmotic hemolysis. Approach to characterization with SFC of native red cells in nonspherical stage is demonstrated.

Paper Details

Date Published: 8 October 2003
PDF: 8 pages
Proc. SPIE 5141, Diagnostic Optical Spectroscopy in Biomedicine II, (8 October 2003); doi: 10.1117/12.500632
Show Author Affiliations
Konstantin A. Semianov, Design Technological Institute of Computer Engineering (Russia)
Peter Alexandrovich Tarasov, Institute of Chemical Kinetics and Combustion (Russia)

Published in SPIE Proceedings Vol. 5141:
Diagnostic Optical Spectroscopy in Biomedicine II
Georges A. Wagnieres, Editor(s)

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