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Proceedings Paper

Time-resolved multifocal multiphoton microscopy
Author(s): Sandrine Leveque-Fort; J. Penon; Marie-Pierre Fontaine-Aupart; Gerard Roger; Patrick Georges
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Paper Abstract

Two photon microscopy is a powerful tool for cells or tissues imaging. However it presents the drawback of being a laser-scanning technique leading to long time acquisition for 3D images. To preserve biological samples from too long experiments and provide a more complete spectroscopic tool we developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition and gives both intensity and lifetime images for all multifocal points.

Paper Details

Date Published: 9 October 2003
PDF: 7 pages
Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.500627
Show Author Affiliations
Sandrine Leveque-Fort, Lab. de Photophysique Moleculaire, CNRS-Univ. Paris Sud (France)
Lab. de Photophysique Moleculaire, CNRS (France)
J. Penon, Lab. de Photophysique Moleculaire, CNRS-Univ. Paris Sud (France)
Lab. de Photophysique Moleculaire, CNRS (France)
Marie-Pierre Fontaine-Aupart, Lab. de Photophysique Moleculaire, CNRS-Univ. Paris Sud (France)
Lab. de Photophysique Moleculaire, CNRS (France)
Gerard Roger, Institut d'Optique (France)
Patrick Georges, Institut d'Optique (France)


Published in SPIE Proceedings Vol. 5139:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging
Tony Wilson, Editor(s)

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