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Proceedings Paper

Local amplitude and phase retrieval method for digital holography applied to microscopy
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Paper Abstract

We present a numerical two-step reconstruction procedure for digital off-axis Fresnel holograms. First, we retrieve the amplitude and phase of the object wave in the CCD plane. For each point we solve a weighted linear set of equations in the least-squares sense. The algorithm has O(N) complexity and gives great flexibility. Second, we numerically propagate the obtained wave to achieve proper focus. We apply the method to microscopy and demonstrate its suitability for the real time imaging of biological samples.

Paper Details

Date Published: 14 October 2003
PDF: 5 pages
Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); doi: 10.1117/12.500517
Show Author Affiliations
Michael Liebling, Ecole Polytechnique Federale de Lausanne (Switzerland)
Thierry Blu, Ecole Polytechnique Federale de Lausanne (Switzerland)
Etienne Cuche, Ecole Polytechnique Federale de Lausanne (Switzerland)
Pierre Marquet, Univ. de Lausanne (Switzerland)
Christian D. Depeursinge, Ecole Polytechnique Federale de Lausanne (Switzerland)
Michael Unser, Ecole Polytechnique Federale de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 5143:
Novel Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara, Editor(s)

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