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Proceedings Paper

Time-resolved multiphoton imaging of the interaction between the PKC and the NFκB signalling pathways
Author(s): Penny E. Morton; Tony Ng; Sarah A. Roberts; Borivoj Vojnovic; Simon M. Ameer-Beg
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Paper Abstract

Using fluorescence resonance energy transfer (FRET) measured by fluorescence lifetime imaging microscopy (FLIM) we have explored the protein-protein interactions between fluorescent protein tagged fusion proteins of the activation pathways of PKC and NFkB. We observe FRET between CFP-IκB and YFP-p65 in unstimulated cells and when treated with TNFα. We also observed a reduction of the fluorescent lifetime of CFP-IκB in the absence of YFP-p65 when TNFα is present.

Paper Details

Date Published: 9 October 2003
PDF: 7 pages
Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.500513
Show Author Affiliations
Penny E. Morton, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Mt. Vernon Hospital (United Kingdom)
King's College London (United Kingdom)
Tony Ng, King's College London (United Kingdom)
Sarah A. Roberts, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Mt. Vernon Hospital (United Kingdom)
Borivoj Vojnovic, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Mt. Vernon Hospital (United Kingdom)
Simon M. Ameer-Beg, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Mt. Vernon Hospital (United Kingdom)


Published in SPIE Proceedings Vol. 5139:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging
Tony Wilson, Editor(s)

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