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Proceedings Paper

Adaptive control of two-photon fluorescence from green fluorescent protein by shaped femtosecond excitation pulses
Author(s): Hiroyuki Kawano; Yasuo Nabekawa; Akira Suda; Yu Oishi; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa
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Paper Abstract

Two-photon excited fluorescence process of green fluorescent protein was adaptively controlled by shaped femtosecond excitation laser pulses. The phase control was given by a fused-silica spatial light modulator. We could completely intentionally increase and decrease of fluorescence efficiency against the excitation intensity. This fully-controllable method can become a robust solution for various practical problems such as photobleaching of the samples on a two-photon fluorescence microscopy.

Paper Details

Date Published: 14 October 2003
PDF: 8 pages
Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); doi: 10.1117/12.500445
Show Author Affiliations
Hiroyuki Kawano, RIKEN-The Institute of Physical and Chemical Research (Japan)
Yasuo Nabekawa, RIKEN-The Institute of Physical and Chemical Research (Japan)
Akira Suda, RIKEN-The Institute of Physical and Chemical Research (Japan)
Yu Oishi, RIKEN-The Institute of Physical and Chemical Research (Japan)
Hideaki Mizuno, RIKEN-The Institute of Physical and Chemical Research (Japan)
Atsushi Miyawaki, RIKEN-The Institute of Physical and Chemical Research (Japan)
Katsumi Midorikawa, RIKEN-The Institute of Physical and Chemical Research (Japan)


Published in SPIE Proceedings Vol. 5143:
Novel Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara, Editor(s)

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