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Proceedings Paper

Unzipping force analysis of protein association (UFAPA): a novel technique to probe protein-DNA interactions
Author(s): Michelle D Wang; Steven J. Koch; Alla Shundrovsky; Benjamin C. Jantzen
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Paper Abstract

We present Unzipping Force Analysis of Protein Association (UFAPA) as a novel and versatile method for detection of the position and dynamic nature of protein-DNA interactions. A single DNA double helix was unzipped in the presence of DNA-binding proteins using a feedback-enhanced optical trap. When the unzipping fork in a DNA reached a bound protein molecule, we observed a dramatic increase in the tension in the DNA, followed by a sudden tension reduction. Analysis of the unzipping force throughout an unbinding "event" revealed information about the spatial location and dynamic nature of the protein-DNA complex.

Paper Details

Date Published: 30 April 2003
PDF: 6 pages
Proc. SPIE 5110, Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems, (30 April 2003); doi: 10.1117/12.500332
Show Author Affiliations
Michelle D Wang, Cornell Univ. (United States)
Steven J. Koch, Cornell Univ. (United States)
Alla Shundrovsky, Cornell Univ. (United States)
Benjamin C. Jantzen, Cornell Univ. (United States)

Published in SPIE Proceedings Vol. 5110:
Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems
Sergey M. Bezrukov; Hans Frauenfelder; Frank Moss, Editor(s)

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