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Proceedings Paper

Comparison of human colorectal normal tissue with cancerous tissue autofluorescence image by optical sectioning with a confocal laser-scanning microscope
Author(s): Sheng Fu; Teck-Chee Chia; Leong Chuan Kwek; Cheong Hoong Diong; Choong Leong Tang; Francis Seow Choen; Shankar Muthu Krishnan
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Paper Abstract

We investigated normal and cancerous human colorectal tissues (fresh thick biopsy specimens) using Olympus Confocal laser scanning biological microscope (FV300). The different layers of autofluorescence images of the specimen were captured by 488 nm laser scanning and sectioning. Optical sectioning can be performed in the vertical plane. Laser scanning can be performed in the horizontal plane. By comparing the autofluorescence image of the normal colorectal tissue with cancerous tissue, the structures of the optical sectioning image layer were found to be significantly different. We have also obtained fibrous autofluorescence image inside tissue specimen. Our investigation may help provide some useful insight to other autofluorescence research studies like laser induced autofluorescence spectra of human colorectal tissue study as a diagnosis technique for clinical application.

Paper Details

Date Published: 9 October 2003
PDF: 7 pages
Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.500203
Show Author Affiliations
Sheng Fu, Nanyang Technological Univ. (Singapore)
Teck-Chee Chia, Nanyang Technological Univ. (Singapore)
Leong Chuan Kwek, Nanyang Technological Univ. (Singapore)
Cheong Hoong Diong, Nanyang Technological Univ. (Singapore)
Choong Leong Tang, Nanyang Technological Univ. (Singapore)
Francis Seow Choen, Nanyang Technological Univ. (Singapore)
Shankar Muthu Krishnan, Singapore General Hospital (Singapore)

Published in SPIE Proceedings Vol. 5139:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging
Tony Wilson, Editor(s)

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