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Proceedings Paper

A cross-correlated experimental setup for probing normal and abnormal vital processes that are endogenous or induced by xenobiotic agents
Author(s): Marziale Milani; Monica Ballerini; Lorenzo Ferraro; Maja Berden Zrimec; Alexis Zrimec; Luka Drinovec; Larissa Brizhik; Federica Causa; Amanda Oldani; Antonio Pasinetti; Massimo Barberis; Maria Cannone
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Paper Abstract

A wealth of data point to Delayed Luminescence (DL) as a good candidate for early and reliable detection technique in neoplastic cells and tissues sorting. Aiming at a DL experimental set up for such a kind of information, a testing technique for morphological analysis should be provided. This could certify the early identification of pathologies and abnormalities in cells and tissues by DL. DL technique may be coupled with FIB (Focused Ion Beam) imaging analysis to give a correlated, both spectroscopic and morphological investigation, at the submicron scale. A strong link among others has been reported to exist between DL signal characteristics and cytoskeleton structure and dynamics: FIB (Focused Ion Beam) imaging is for the moment being the best non invasive check at all and it can detect morphological alterations as early as possible since its resolution can go down to 2-5 nm. The cells, that can be highlighted by the fast DL and slow and efficient FIB, can be in parallel analysed by a metabolic manometric technique that uses differential pressure sensors: the different cellular activity of normal and abnormal cells can be recorded and this allows fast and non-invasive investigations, although requiring a minimal number of cells. In addition it’s possible to study, by the confocal microscopy spectroscopic analysis, DNA fragments, exploiting the optical characteristics of a dye, like ethidium bromide, to detect dynamic and conformational changes in DNA chains. These changes can be artificially induced in cells (e.g. by irradiation) or found in neoplastic cells. The acquired experience allows an independent check of spectroscopic, morphologic and metabolic testing by a control on nucleic acid defects. These four techniques may be used together creating a "protocol" in order to permit an early and reliable alterations diagnosis of cells and tissues, guaranteeing an high accuracy standard.

Paper Details

Date Published: 14 October 2003
PDF: 12 pages
Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); doi: 10.1117/12.500201
Show Author Affiliations
Marziale Milani, Univ. degli Studi di Milano-Bicocca (Italy)
Monica Ballerini, Univ. degli Studi di Milano-Bicocca (Italy)
Consorzio Milano Ricerche (Italy)
Lorenzo Ferraro, Univ. degli Studi di Milano-Bicocca (Italy)
Maja Berden Zrimec, Institute BION (Slovenia)
Alexis Zrimec, Institute of Physical Biology (Slovenia)
Luka Drinovec, Institute of Physical Biology (Slovenia)
Larissa Brizhik, Bogolyubov Institute for Theoretical Physics (Ukraine)
Federica Causa, Univ. of Bath (United Kingdom)
Amanda Oldani, Univ. degli Studi di Milano-Bicocca (Italy)
Consorzio Milano Ricerche (Italy)
Antonio Pasinetti, Univ. degli Studi di Milano (Italy)
Massimo Barberis, Policlinico Multimedica (Italy)
Maria Cannone, Policlinico Multimedica (Italy)

Published in SPIE Proceedings Vol. 5143:
Novel Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara, Editor(s)

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