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Proceedings Paper

Confocal microscopy in transmitted light
Author(s): Hans-Ulrich Dodt; Klaus Becker
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Paper Abstract

We developed a confocal microscope for transmitted light to visualize fine details in phase objects like unstained biological specimens. The main difficulty of confocal microscopy in transmission is the alignment of illumination and detector pinholes. This alignment was achieved by using "electronic pinholes" on the detector side. As a first step, we were able to image cells in onion skin at greater depths and with higher resolution than by using conventional microscopy.

Paper Details

Date Published: 9 October 2003
PDF: 9 pages
Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.499676
Show Author Affiliations
Hans-Ulrich Dodt, Max-Planck-Institut fuer Psychiatrie (Germany)
Klaus Becker, Max-Planck-Institute fuer Psychiatry (Germany)


Published in SPIE Proceedings Vol. 5139:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging
Tony Wilson, Editor(s)

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