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Proceedings Paper

CARS microscopy with dark field excitation
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Paper Abstract

We use coherent anti-Stokes Raman scattering (CARS) for functional imaging in microscopy. In contrast to other methods, excitation and detection are performed in a wide-field (non-confocal) setup, similar to a combination of dark field and epi-fluorescence microscopy. Thus, imaging of the whole sample is performed at once, i.e. without scanning, which promises the possibility of fast microscopy with vibrational contrast. The use of a nanosecond laser system rather than typically used pico- or femtosecond systems facilitates a high spectroscopic resolution for various organic substances.

Paper Details

Date Published: 9 October 2003
PDF: 10 pages
Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.499634
Show Author Affiliations
Christoph Heinrich, Univ. of Innsbruck (Austria)
Stefan Bernet, Univ. of Innsbruck (Austria)
Monika Ritsch-Marte, Univ. of Innsbruck (Austria)

Published in SPIE Proceedings Vol. 5139:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging
Tony Wilson, Editor(s)

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