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Proceedings Paper

Spatial structure of chromatin in hybrid cells produced by laser-induced fusion studied by optical microscopy
Author(s): Jan Jezek; Stanislav Palsa; Emilie Lukasova; Stanislav Kozubek; Petr Jakl; Mojmir Sery; Alexandr Jonas; Miroslav Liska; Pavel Zemanek
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Paper Abstract

In this article we describe a combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to punctuate their membranes at the contact point. This process initiates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The spatial distribution of the nuclear material in the resulting hybrid nucleus is studied by analysis of positions of FISH (fluorescent hybridization in situ) signals of specific genetic loci in automated fluorescence microscope (high resolution cytometer). The obtained results are compared to the signals distribution of FISH in the original cells.

Paper Details

Date Published: 8 July 2003
PDF: 5 pages
Proc. SPIE 5036, Photonics, Devices, and Systems II, (8 July 2003); doi: 10.1117/12.498654
Show Author Affiliations
Jan Jezek, Institute of Scientific Instruments (Czech Republic)
Stanislav Palsa, Institute of Biophysics (Czech Republic)
Emilie Lukasova, Institute of Biophysics (Czech Republic)
Stanislav Kozubek, Institute of Biophysics (Czech Republic)
Petr Jakl, Institute of Scientific Instruments (Czech Republic)
Mojmir Sery, Institute of Scientific Instruments (Czech Republic)
Brno Univ. of Technology (Czech Republic)
Alexandr Jonas, Institute of Scientific Instruments (Czech Republic)
Miroslav Liska, Brno Univ. of Technology (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments (Czech Republic)


Published in SPIE Proceedings Vol. 5036:
Photonics, Devices, and Systems II
Miroslav Hrabovsky; Dagmar Senderakova; Pavel Tomanek, Editor(s)

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