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Proceedings Paper

New two-photon fluorescent probe for multiphoton microscopy in biological media
Author(s): Raz Gvishi; Garry Berkovic; Zvi Kotler; P. Krief; J. Y. Becker; M. Sigalov; Lev Shapiro; Vladimir Khodorkovsky
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Paper Abstract

An important ingredient in improving Multi Photon Laser Scanning Microscopy, MPLSM, is the development of efficient two-photon fluorescent (TPF) probes. We previously reported on a new class of TPF probes, specifically designed in order to maximize their efficiency in potential MPLSM applications. The fluorophores are based on a tetraketo derivative (TK) with a symmetric structure Donor-Acceptor-Donor (D-A-D). Those fluorophores have the following properties: a) Very large two-photon absorption coefficients (δ ~ 1000GM); b) Two-photon excitation (TPE) peak wavelength strongly shifted to the red (λ ~ 1µm); c) High fluorescence quantum efficiency; d) Large Stokes shifts of the fluorescence bands. We extended our work to a new fluorophore from this class that is more suitable for biological settings. This new fluorophore has a structure of crown-TK-crown that incorporates the ability to trap metal ions such as calcium. The TPE wavelength dependence of the TK-crown derivative is very similar to its analogous linear derivative with enhancement in the value of the cross-section, due to the stronger donor moieties. The TPE cross-section for the TK-crown derivative was about δ = 950 GM at λmax = 980 nm.

Paper Details

Date Published: 8 July 2003
PDF: 6 pages
Proc. SPIE 5036, Photonics, Devices, and Systems II, (8 July 2003); doi: 10.1117/12.498451
Show Author Affiliations
Raz Gvishi, Soreq Nuclear Research Ctr. (Israel)
Garry Berkovic, Soreq Nuclear Research Ctr. (Israel)
Zvi Kotler, Soreq Nuclear Research Ctr. (Israel)
P. Krief, Ben-Gurion Univ. of the Negev (Israel)
J. Y. Becker, Ben-Gurion Univ. of the Negev (Israel)
M. Sigalov, Ben-Gurion Univ. of the Negev (Israel)
Lev Shapiro, Ben-Gurion Univ. of the Negev (Israel)
Vladimir Khodorkovsky, Ben-Gurion Univ. of the Negev (Israel)


Published in SPIE Proceedings Vol. 5036:
Photonics, Devices, and Systems II
Miroslav Hrabovsky; Dagmar Senderakova; Pavel Tomanek, Editor(s)

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