Share Email Print

Proceedings Paper

New two-photon fluorescent probe for multiphoton microscopy in biological media
Author(s): Raz Gvishi; Garry Berkovic; Zvi Kotler; P. Krief; J. Y. Becker; M. Sigalov; Lev Shapiro; Vladimir Khodorkovsky
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

An important ingredient in improving Multi Photon Laser Scanning Microscopy, MPLSM, is the development of efficient two-photon fluorescent (TPF) probes. We previously reported on a new class of TPF probes, specifically designed in order to maximize their efficiency in potential MPLSM applications. The fluorophores are based on a tetraketo derivative (TK) with a symmetric structure Donor-Acceptor-Donor (D-A-D). Those fluorophores have the following properties: a) Very large two-photon absorption coefficients (δ ~ 1000GM); b) Two-photon excitation (TPE) peak wavelength strongly shifted to the red (λ ~ 1µm); c) High fluorescence quantum efficiency; d) Large Stokes shifts of the fluorescence bands. We extended our work to a new fluorophore from this class that is more suitable for biological settings. This new fluorophore has a structure of crown-TK-crown that incorporates the ability to trap metal ions such as calcium. The TPE wavelength dependence of the TK-crown derivative is very similar to its analogous linear derivative with enhancement in the value of the cross-section, due to the stronger donor moieties. The TPE cross-section for the TK-crown derivative was about δ = 950 GM at λmax = 980 nm.

Paper Details

Date Published: 8 July 2003
PDF: 6 pages
Proc. SPIE 5036, Photonics, Devices, and Systems II, (8 July 2003); doi: 10.1117/12.498451
Show Author Affiliations
Raz Gvishi, Soreq Nuclear Research Ctr. (Israel)
Garry Berkovic, Soreq Nuclear Research Ctr. (Israel)
Zvi Kotler, Soreq Nuclear Research Ctr. (Israel)
P. Krief, Ben-Gurion Univ. of the Negev (Israel)
J. Y. Becker, Ben-Gurion Univ. of the Negev (Israel)
M. Sigalov, Ben-Gurion Univ. of the Negev (Israel)
Lev Shapiro, Ben-Gurion Univ. of the Negev (Israel)
Vladimir Khodorkovsky, Ben-Gurion Univ. of the Negev (Israel)

Published in SPIE Proceedings Vol. 5036:
Photonics, Devices, and Systems II
Miroslav Hrabovsky; Dagmar Senderakova; Pavel Tomanek, Editor(s)

© SPIE. Terms of Use
Back to Top