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An integrated software solution for real-time PCR analysis based on microfluidic biochip
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Paper Abstract

In this paper, we present an integrated and automated prototype system which has been developed for real-time polymerase chain reaction (PCR) analysis based on microfluidic PCR array chips. The system integrates the PCR thermal cycling and optical detection capabilities to enable real-time fluorescence imaging and image processing for data analysis. The main advantage of the system is that it provides a solution that can rapidly perform and evaluate PCR experiment simultaneously on microfluidic PCR array chips. The system has demonstrated fast and efficient on-chip real-time PCR analysis using human genomic DNA samples. The implementation of the system integration is a multi-thread Windows software with component structure which is written in Visual C++.

Paper Details

Date Published: 18 April 2003
PDF: 10 pages
Proc. SPIE 5119, Bioengineered and Bioinspired Systems, (18 April 2003); doi: 10.1117/12.498280
Show Author Affiliations
Qinghui Wang, Nanyang Technological Univ. (Singapore)
Haiqing Gong, Nanyang Technological Univ. (Singapore)

Published in SPIE Proceedings Vol. 5119:
Bioengineered and Bioinspired Systems
Angel Rodriguez-Vazquez; Derek Abbott; Ricardo Carmona, Editor(s)

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