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Proceedings Paper

Amplification and modelling of bioaffinity detection with terahertz spectroscopy
Author(s): Samuel P. Mickan; Abdellah Menikh; Jesper Munch; Derek Abbott; Xi-Cheng Zhang
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Paper Abstract

A new bioaffinity sensor based on pulsed terahertz (THz) spectroscopy is able to sensitively detect the presence of ultra-thin bound biomolecular layers. The protein avidin and lipid biotin are noted for their very high binding affinity, and the ease for which they can be attached to residues with importance in many biosensing applications. We demonstrate the sensitivity of the pulsed THz spectrometer to thin avidin layers and to avidin amplified with micron-sized agarose beads. The experimental results can be simply modelled by considering transmission of the THz radiation at the thin film interfaces. We detect less than 10.3 ng/cm2 avidin, giving the THz system a detection capability of sub-thin solid films better than ellipsometry and reflectometry techniques.

Paper Details

Date Published: 14 November 2002
PDF: 9 pages
Proc. SPIE 4937, Biomedical Applications of Micro- and Nanoengineering, (14 November 2002); doi: 10.1117/12.488143
Show Author Affiliations
Samuel P. Mickan, Univ. of Adelaide (Australia)
Rensselaer Polytechnic Institute (United States)
Abdellah Menikh, Rensselaer Polytechnic Institute (United States)
Univ. of Constantinte (Algeria)
Jesper Munch, Univ. of Adelaide (Australia)
Derek Abbott, Univ. of Adelaide (Australia)
Xi-Cheng Zhang, Rensselaer Polytechnic Institute (United States)


Published in SPIE Proceedings Vol. 4937:
Biomedical Applications of Micro- and Nanoengineering
Dan V. Nicolau, Editor(s)

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