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Proceedings Paper

Raman microscopy of cells: chemical imaging of apoptosis
Author(s): Natallia Uzunbajakava; Jan Greve; Cees Otto
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Paper Abstract

Singular value decomposition was applied to the set of the non-resonant Raman spectra, recorded during Raman imaging of the single apoptotic cell. The basis vectors and the corresponding singular values were assessed in terms of their statistical significance. The noise-containing basis vectors were rejected while keeping the meaningful ones. In this way the Raman images of the apoptotic cell were successfully reconstructed from the spectrally-filtered data matrix. The results are demonstrated on the spatial distribution of the DNA, protein and phospholipids, present in the fragments of the apoptotic cell.

Paper Details

Date Published: 10 July 2003
PDF: 8 pages
Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.487609
Show Author Affiliations
Natallia Uzunbajakava, Univ. of Twente (Netherlands)
Jan Greve, Univ. of Twente (Netherlands)
Cees Otto, Univ. of Twente (Netherlands)


Published in SPIE Proceedings Vol. 4963:
Multiphoton Microscopy in the Biomedical Sciences III
Ammasi Periasamy; Peter T. C. So, Editor(s)

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