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Proceedings Paper

Detection of salmonella using surfaced enhanced Raman scattering
Author(s): Joseph R. Montoya; Robert L. Armstrong; Geoffrey B. Smith
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Paper Abstract

The use of Surface Enhanced Raman Scattering (SERS) for biological detection has brought up the question of detection limits and how these detection limits apply to the application. For most biological detection uses of SERS, a high detection probability is needed for a relatively small amount of biological specimen. This is especially true for the detection of S. enteriditis (Salmonella) bacteria that may be present on minute concentrations , for example, in food products. Using SERS we have identified the associated antibody conjugated with 12nm diameter Au colloid. Our preliminary results show small fractals with a disperse distance of about 1 monomer diameter (12nm) between the colloidal gold monomers may enhance the SERS emission. We also investigate the possibility that a conformation change may induce an increase in the aromatic amino acid contribution. We then compare the antibody SERS alone to SERS of antibody conjugated to Salmonella bacteria. The use of SERS as a bacterial detection method leads to the possibility for detection of small amounts (<10,000 bacteria/ml) of Salmonella bacteria. In our study we obtained a detection limit of 106 bacteria/ml using gold as a SERS active substrate.

Paper Details

Date Published: 15 August 2003
PDF: 9 pages
Proc. SPIE 5085, Chemical and Biological Sensing IV, (15 August 2003); doi: 10.1117/12.487144
Show Author Affiliations
Joseph R. Montoya, New Mexico State Univ. (United States)
Robert L. Armstrong, New Mexico State Univ. (United States)
Geoffrey B. Smith, New Mexico State Univ. (United States)

Published in SPIE Proceedings Vol. 5085:
Chemical and Biological Sensing IV
Patrick J. Gardner, Editor(s)

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