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Proceedings Paper

Formation of resting cells by non-spore-forming microorganisms as a strategy of long-term survival in the environment
Author(s): Andrei L. Mulyukin; Vera S. Soina; Elena V. Demkina; Alla N. Kozlova; Natalia E. Suzina; Vladimir V. Dmitriev; Vitalii I. Duda; Galina I. El'-Registan
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Paper Abstract

Non-spore-forming bacteria of the genera Micrococcus and Arthrobacter, including the isolates from permafrost sediments, were found to be able to form cystlike cells under special conditions. Cystlike cells maintained the viability during long-term storage (for up to several years), had undetectable respiratory activity and the elevated resistance to heating and other unfavorable conditions, possessed the specific fine structure and morphology, and were formed in the life cycles of the microorganism. These properties allow cystlike cells to be attributed to a new type of resting microbial forms. Furthermore, the distinctive feature of resting cystlike cells was their low P/S ratios and high Ca/K ratios in comparison to vegetative cells as shown by X-ray microanalysis. The experimentally obtained bacterial cystlike cells with thickened and laminated cell walls and altered texture of the cytoplasm were similar to the cells abundant in native microbial populations isolated from permafrost sediments and ancient soils of the Kolyma lowland (Siberia, Russia). Due to the inherent elevated resistance to adverse conditions and maintenance of viability for prolonged periods, resting cystlike cells are likely to ensure long-term survival of non-spore-forming bacteria in cold environments.

Paper Details

Date Published: 30 January 2003
PDF: 11 pages
Proc. SPIE 4939, Instruments, Methods, and Missions for Astrobiology VI, (30 January 2003); doi: 10.1117/12.486686
Show Author Affiliations
Andrei L. Mulyukin, Institute of Microbiology (Russia)
Vera S. Soina, Moscow State Univ. (Russia)
Elena V. Demkina, Moscow State Univ. (Russia)
Alla N. Kozlova, Institute of Microbiology (Russia)
Natalia E. Suzina, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms (Russia)
Vladimir V. Dmitriev, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms (Russia)
Vitalii I. Duda, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms (Russia)
Galina I. El'-Registan, Institute of Microbiology (Russia)


Published in SPIE Proceedings Vol. 4939:
Instruments, Methods, and Missions for Astrobiology VI
Richard B. Hoover; Alexei Yu. Rozanov; Jere H. Lipps, Editor(s)

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