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Proceedings Paper

Monitoring of DsRed protein concentration in frozen insect larvae
Author(s): Yordan Kostov; Leah Tolosa; Kevin O'Connell; Patricia Anderson; Yi Liu; Nikolai van Beek; Govind Rao
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Paper Abstract

A baculovirus expression system was used to produce DsRed fusion protein in insect larvae. As the baculovirus/insect larvae system requires precise harvest timing to achieve high yield of protein, a low-cost miniature all-solid state optical probe was used for detection of the protein concentrations in the frozen larvae. Three batches of infected larvae were monitored at different post-infection times. The calibration curve of the probe was obtained by simultaneous measurements both in laboratory fluorimeter and using gel electrophoresis analysis. The results show good correlation between the optical measurements and the standard laboratory technique.

Paper Details

Date Published: 12 September 2003
PDF: 8 pages
Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); doi: 10.1117/12.485720
Show Author Affiliations
Yordan Kostov, Univ. of Maryland, Baltimore County (United States)
Leah Tolosa, Univ. of Maryland, Baltimore County (United States)
Kevin O'Connell, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Patricia Anderson, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Yi Liu, Chesapeak PERL, Inc. (United States)
Nikolai van Beek, Chesapeak PERL, Inc. (United States)
Govind Rao, Univ. of Maryland, Baltimore County (United States)


Published in SPIE Proceedings Vol. 4967:
Genetically Engineered and Optical Probes for Biomedical Applications
Darryl J. Bornhop; Alexander P. Savitsky; Ramesh Raghavachari; Samuel I. Achilefu, Editor(s)

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