Share Email Print
cover

Proceedings Paper

All-optical thrombotic stroke model for near-surface blood vessels in rat: focal illumination of exogeneous photosensitizers combined with real-time two-photon imaging
Author(s): Christopher B. Schaffer; Philbert S. Tsai; Nozomi Nishimura; Lee F. Schroeder; Beth Friedman; Patrick D. Lyden; Ford F. Ebner; David Kleinfeld
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Photothrombotic microstrokes are produced in rat cortex by 532-nm single-photon optical excitation of an intravenously injected photosensitizer, rose bengal. The dynamics of blood flow and clot formation in the cortical vasculature are observed using two-photon laser scanning microscopy of an intravenously injected fluorescent dye. Flowing and clotted vessels are clearly distinguishable in both large and small vessels, down to individual capillaries, using this technique. We find that by tightly focusing the laser light used to excite the photosensitizer, clots can be formed in individual blood vessels without affecting neighboring vessels tens of micrometers away. We observe many changes in blood flow as a result of localized clot formation, including upstream vascular dilation, clot clearing, i.e. recanalization, and complete reversal of blood flow direction downstream.

Paper Details

Date Published: 19 June 2003
PDF: 10 pages
Proc. SPIE 4978, Commercial and Biomedical Applications of Ultrafast Lasers III, (19 June 2003); doi: 10.1117/12.484208
Show Author Affiliations
Christopher B. Schaffer, Univ. of California/San Diego (United States)
Philbert S. Tsai, Univ. of California/San Diego (United States)
Nozomi Nishimura, Univ. of California/San Diego (United States)
Lee F. Schroeder, Univ. of California/San Diego (United States)
Beth Friedman, Univ. of California/San Diego (United States)
Patrick D. Lyden, Univ. of California/San Diego (United States)
Ford F. Ebner, Vanderbilt Univ. (United States)
David Kleinfeld, Univ. of California/San Diego (United States)


Published in SPIE Proceedings Vol. 4978:
Commercial and Biomedical Applications of Ultrafast Lasers III
Joseph Neev; Andreas Ostendorf; Christopher B. Schaffer, Editor(s)

© SPIE. Terms of Use
Back to Top