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Proceedings Paper

Immobilization of multiple proteins in polymer microstructures fabricated via laser ablation
Author(s): Elena P. Ivanova; Andrea Viezzoli; Yulia V. Alekseeva; Gregory M. Demyashev; Dan Valeriu Nicolau; Luisa Filipponi; Duy K. Pham; Dan V. Nicolau
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Paper Abstract

Investigation of protein-polymeric surface interaction requires reliable practical techniques for evaluation of the efficiency of protein immobilization. In this study the efficiency of protein immobilization was evaluated using three different techniques: (1) protein-binding assay with fluorescent detection and (2) quantification, and (3) atomic force microscopy. This approach enables us to rapidly analyse the adsorption properties of different proteins. The comparative physico-chemical adsorption of α-chymotrypsin, human serum albumin, human immunoglobulin, lysozyme, and myoglobin in the micro-wells fabricated via a localized laser ablation of a protein-blocked thin gold layer (50 nm) deposited on a Poly(methyl ethacrylate) film has been studied. Correlations were observed between the quantitative and qualitative differences depending on both protein and polymeric surface hydrophobicity.

Paper Details

Date Published: 18 July 2003
PDF: 13 pages
Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); doi: 10.1117/12.483472
Show Author Affiliations
Elena P. Ivanova, Swinburne Univ. of Technology (Australia)
Andrea Viezzoli, Swinburne Univ. of Technology (Australia)
Yulia V. Alekseeva, Swinburne Univ. of Technology (Australia)
Gregory M. Demyashev, Swinburne Univ. of Technology (Australia)
Dan Valeriu Nicolau, Swinburne Univ. of Technology (Australia)
Luisa Filipponi, Swinburne Univ. of Technology (Australia)
Duy K. Pham, Swinburne Univ. of Technology (Australia)
Dan V. Nicolau, Swinburne Univ. of Technology (Australia)


Published in SPIE Proceedings Vol. 4966:
Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis
Dan V. Nicolau; Ramesh Raghavachari, Editor(s)

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