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Proceedings Paper

Rapid imaging of oxygen free radicals produced in PDT using FCLA chemiluminescence probe
Author(s): Da Xing; Juan Wang; Yonghong He
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Paper Abstract

In this paper, we report a rapid imaging method for oxygen free radicals produced in photodynamic therapy (PDT) in vivo using chemiluminescence probe. The mechanism for PDT involves singlet oxygen (102)generated by energy transfer from photosensitizers. '02 can react with FCLA, which is a specific chemiluminescence probe for detecting 102 and superoxide (02). The reaction of FCLA (Fluoresceinyl Cypridina Luminescent Analog) and 102can give emission with peak wavelength at about 532 nm. In the present study, FCLA was chosen as an optical reporter of 102produced from photosensitization reaction of Hematoporphyrin Derivative (HpD) in model solution and in nude mice with transplanted mammary cancer. Photosensitized chemiluminescence from the reaction of FCLA with 102was detected by a highly sensitive Intensified Charge-Coupled Device (ICCD) detector. The chemiluminescence was markedly inhibited by the addition of 10 mmol/L sodium azide (NaN3) to the model solution and minor effects were observed at the addition of 1 0 imol/L superoxide dismutase (SOD), 20 mmol/L mannitol and I 00 tg/mL catalase, respectively, thus indicating that '02 generation from photosensitization reaction mainly results in the light emission. Experiments in vivo with tumor-bearing mouse showed a clear chemiluminescence image of tumor.

Paper Details

Date Published: 12 September 2002
PDF: 9 pages
Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); doi: 10.1117/12.482963
Show Author Affiliations
Da Xing, South China Normal Univ. (China)
Juan Wang, South China Normal Univ. (China)
Yonghong He, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 4916:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment
Britton Chance; Mingzhe Chen; Gilwon Yoon, Editor(s)

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