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Proceedings Paper

Highly sensitive chemiluminescence analysis system for medical radiation dose evaluation
Author(s): Xian Li; Yadong Wei; Da Xing; Yonghong He
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Paper Abstract

Chemiluminescence (CL) analysis technique is an important method in biological and medical diagnosis. In this research work, we present a novel system to detect and analyze the CL during the actinotheraphy to evaluate the radiation dose according to the principle of the cell stress response to ionizing radiation. The system consists of a sample chamber, a photomultiplier tube (PMT) detector, an amplifier, a discriminator, and a pulses counter. The PMT was cooled to reduce the thermal noise. With the single photon counting technique, high sensitivity, signal-to-noise ratio and stability was achieved. The sample chamber, which was driven by a step motor, has six sample cells for control experiments. A personal computer dominates the data acquisition and the control of the system through the newly implemented universal serial bus interface. There is also a peristaltic pump for auto-injecting the chemical reagent solution. The software developed on the LabVIEW platform makes the system with high automation. With the system, radiation dose could be quantified by the CL intensity from the cell stress response to ionizing radiation. Leukocytes, CCRF cell line and a CL probe MCLA were used to test the reliability ofthis system. The relationship between the 60Co irradiation doses and the intensity ofthe MCLA CL were obtained, which showed that this system could image the CL properly.

Paper Details

Date Published: 12 September 2002
PDF: 6 pages
Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); doi: 10.1117/12.482943
Show Author Affiliations
Xian Li, South China Normal Univ. (China)
Yadong Wei, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Yonghong He, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 4916:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment
Britton Chance; Mingzhe Chen; Gilwon Yoon, Editor(s)

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