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Proceedings Paper

Computer analysis method for determining protein relative molecular weight in gel electrophoresis images
Author(s): Sixiang Zhang; Weiling Liu; Duogang Ran; Jiancai Wang; Xiaoxiao Li
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Paper Abstract

The technology of computer analysis for gel electrophoresis images is based on the computer image analysis and gel electrophoresis. Particle in complex mixture have different molecular weight and with different charge. Migration distances of sample zones are compared with the position of standards and the molecular size of unknowns is determined. By this way, biology particle and molecule with different compound can be separated from other. Digital camera can acquire image and process digital transact, then based on the digital image to realize automatic identification, sign, molecular weight calculate. For the convenient of observe and acquire, sample pre band on ethidium bromide stained agarose gels. The instrument is mainly made up of UV light source, digital camera and camera obscura etc. Formulas for calculate of protein's molecular weight and mobility in polyacrylamide gel is connected with standard curve and the relative mobility of band. After many experiments the conclusion can be deduced that the change of electrophoresis start point and front edge (m, n, r's value) will result in the change of K and b. Unknown samples' molecular weight only have relation with the standard sample's molecular weight and the value of r1, r2 and r3.

Paper Details

Date Published: 12 September 2002
PDF: 5 pages
Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); doi: 10.1117/12.482926
Show Author Affiliations
Sixiang Zhang, Hebei Univ. of Technology (China)
Weiling Liu, Hebei Univ. of Technology (China)
Duogang Ran, Hebei Univ. of Technology (China)
Jiancai Wang, Hebei Univ. of Technology (China)
Xiaoxiao Li, Hebei Univ. of Technology (China)

Published in SPIE Proceedings Vol. 4916:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment
Britton Chance; Mingzhe Chen; Gilwon Yoon, Editor(s)

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