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Proceedings Paper

Assessing changes in physical lens dimensions during immersion in tissue preservation media using shadowphotogrammetry
Author(s): Alexander Rosen; David B. Denham; Fabrice Manns; Viviana Fernandez; Joseph Stoiber; Robert Augusteyn; Jean-Marie A. Parel
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Paper Abstract

Purpose: To develop a technique to assess isotonic solutions for crystalline lens preservation. Methods: BSS, Ringer’s, Dulbecco’s Modified Eagle Medium (DMEM), and TC 199 have been selected for experimentation. Donor human cadaver lenses are extracted by cutting the zonules and vitreous adherences. A custom-made testing cell and modified optical comparator are used for imaging, and the lens diameter and thickness profiles are measured using digital photography and a graphics program. Measurements are taken every 5 minutes for the 1st hour and then every 15 minutes for 4 hrs. The volume of the lens at each interval is approximated, assuming rotational symmetry, by using the equation for the volume of an ellipsoid. The changes in diameter, thickness, and volume versus time are analyzed to compare the effect of each solution. Results: The measurement resolution for the digital technique is 13μm. Lens thickness changed more significantly than diameter and volume. All four solutions produced similar preliminary results with maximum diameter, thickness, and volume changes of approximately -2%, +6%, and +3%, respectively. Conclusion: Shadowphotogrammetry can measure the lens physical dimensions to +/-13um and can be used to determine the effect of preservation media. This technique may be useful for assessing the physical effects of chemical and biological substances on the lens osmotic transport system.

Paper Details

Date Published: 14 July 2003
PDF: 6 pages
Proc. SPIE 4951, Ophthalmic Technologies XIII, (14 July 2003); doi: 10.1117/12.482374
Show Author Affiliations
Alexander Rosen, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Univ. of Miami (United States)
David B. Denham, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Univ. of Miami (United States)
Fabrice Manns, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Univ. of Miami (United States)
Viviana Fernandez, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Univ. of Valle School of Medicine (Colombia)
Joseph Stoiber, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Landesklinik fuer Augenheilkunde und Optometrie (United States)
Robert Augusteyn, National Vision Research Institute (Australia)
Jean-Marie A. Parel, Bascom Palmer Eye Institute, Univ. of Miami (United States)
Univ. of Miami (United States)
Univ. de Liege CHO Sart-Tilman (Belgium)


Published in SPIE Proceedings Vol. 4951:
Ophthalmic Technologies XIII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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