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Proceedings Paper

Imaging of tumors by time-delayed laser-induced fluorescence
Author(s): M. Kohl; Joerg Neukammer; Uwe Sukowski; Herbert H. Rinneberg; Hanns-joerg Sinn; E. A. Friedrich; Georgi Graschew; Peter M. Schlag; Dieter Woehrle
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Paper Abstract

A technique to improve signal-to-background ratio in fluorescence images of superficially growing tumors marked with photosensitizers is described. Time-delayed detection of fluorescence following pulsed-laser excitation allows suppression of the autofluorescence background falling into the fluorescence band of the photosensitizer. This technique exploits the longer fluorescence-decay times of porphyrin-based photosensitizers compared to average decay times of tissue autofluorescence. The feasibility of time-delayed fluorescence imaging of tumors has been demonstrated in vitro. From time-delayed fluorescence spectra the authors infer that the ratio between photosensitizer signal and autofluorescence background can be improved by about one order of magnitude.

Paper Details

Date Published: 1 November 1991
PDF: 9 pages
Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); doi: 10.1117/12.48208
Show Author Affiliations
M. Kohl, Physikalisch-Technische Bundesanstalt (Germany)
Joerg Neukammer, Physikalisch-Technische Bundesanstalt (Germany)
Uwe Sukowski, Physikalisch-Technische Bundesanstalt (Germany)
Herbert H. Rinneberg, Physikalisch-Technische Bundesanstalt (Germany)
Hanns-joerg Sinn, Deutsches Krebsforschungszentrum (Germany)
E. A. Friedrich, Deutsches Krebsforschungszentrum (Germany)
Georgi Graschew, Deutsches Krebsforschungszentrum (Germany)
Peter M. Schlag, Chirurgische Universitaetsklinik Heidelberg (Germany)
Dieter Woehrle, Univ. Bremen (Germany)

Published in SPIE Proceedings Vol. 1525:
Future Trends in Biomedical Applications of Lasers
Lars Othar Svaasand, Editor(s)

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