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Proceedings Paper

Single-molecule detection system of optical tweezers
Author(s): Hongbin T. Chen; Y. M. Li; L. R. Lou; Zan Gong
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Paper Abstract

Using optical tweezers we have developed a biomechanical detection system for single molecules detection. This system can control the sample bead which is used as the handle of single molecules with nanometer precision, it also can make quantitative measurements of displacement with nanometer resolution, and measure the Pico-Newton force involved in the dynamical process of the movement of a single biomolecule. We combined two Donut-Mode optical traps with two high-precision observation setups in this system. Experiments described in this paper demonstrate the performance of the system. In our experiments, we monitored the Brownian motion ofpolystyrene beads; as a result we can calculate the optical-trap stiffness. In summary, the system can be used in the research of the dynamical motion of a single biomolecule.

Paper Details

Date Published: 5 September 2002
PDF: 8 pages
Proc. SPIE 4923, Nano-Optics and Nano-Structures, (5 September 2002); doi: 10.1117/12.481712
Show Author Affiliations
Hongbin T. Chen, Univ. of Science & Technology of China (China)
Y. M. Li, Univ. of Science and Technology of China (China)
L. R. Lou, Univ. of Science and Technology of China (China)
Zan Gong, Univ. of Science and Technology of China (China)

Published in SPIE Proceedings Vol. 4923:
Nano-Optics and Nano-Structures
Xing Zhu; Stephen Y. Chou; Yasuhiko Arakawa, Editor(s)

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