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Proceedings Paper

Polymer-based microfluidic devices for biomedical applications
Author(s): Mateusz L. Hupert; Malgorzata A. Witek; Yun Wang; Michael W. Mitchell; Xuezhu Liu; Yannick Bejat; Dimitris E. Nikitopoulos; Jost Goettert; Michael C. Murphy; Steven A. Soper
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Paper Abstract

Two types of Microfluidic bioanalytical systems were designed and fabricated in polymer substrates using the LIGA process. A continuous flow polymerase chain reaction (CFPCR) Microfluidic device was fabricated in polycarbonate (PC), which utilized isothermal zone and shuttling the sample through each zone to achieve amplification. A 20-cycle PCR amplification of a fragment of a plasmid DNA template was achieved in 5.3 min. The results were comparable to those obtained in commercial laboratory-scale PCR system. The second system consisted of a microchip contating a low-density array assembled into the Microfluidic channel, which was hot-embossed in poly(methyl methacrylate) (PMMA). The detection of low-abundant mutations in gene fragments (K-ras) that carry point mutations with high diagnostic value for colorectal cancer was successfully performed. The array accessed microfluidics in order to enhance the kinetic associated with hybridization.

Paper Details

Date Published: 17 January 2003
PDF: 13 pages
Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); doi: 10.1117/12.480771
Show Author Affiliations
Mateusz L. Hupert, Louisiana State Univ. (United States)
Malgorzata A. Witek, Louisiana State Univ. (United States)
Yun Wang, Louisiana State Univ. (United States)
Michael W. Mitchell, Louisiana State Univ. (United States)
Xuezhu Liu, Louisiana State Univ. (United States)
Yannick Bejat, Louisiana State Univ. (United States)
Dimitris E. Nikitopoulos, Louisiana State Univ. (United States)
Jost Goettert, Louisiana State Univ. (United States)
Michael C. Murphy, Louisiana State Univ. (United States)
Steven A. Soper, Louisiana State Univ. (United States)


Published in SPIE Proceedings Vol. 4982:
Microfluidics, BioMEMS, and Medical Microsystems
Holger Becker; Peter Woias, Editor(s)

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