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Proceedings Paper

High-throughput screening system for the study of phototoxicity of photosensitizers in vitro
Author(s): Irina G. Meerovich; Victoria V. Jerdeva; Gennadii A. Meerovich; Valentina M. Derkacheva; Alexander P. Savitsky
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Paper Abstract

The technique and the device for studying of phototoxic properties of photosensitizers in vitro on cell monolayers in 96-well microplate was developed. It allows to irradiate independently each well of microtiter plate, and study simultaneously all points of dependence of phototoxic effect on the light dose for certain conditions of investigation. Also it allows to study and compare at the same conditions the dependence of phototoxic effect on the light dose for different concentrations of photosensitizer or for several photosensitizers. Developed device includes powerful source of light based arc xenon lamp with elliptical reflector, special filters and multi-fiber bundle. To determine the degree of the cell viability under the photodynamic treatment proliferative test with fluoresceinediacetate was used. Using this device and technique phototoxic action of a number of different derivatives of aluminium phthalocyanines was studied. The results of this screening have a good correlation with the results obtained in vivo on mice.

Paper Details

Date Published: 13 June 2003
PDF: 6 pages
Proc. SPIE 4952, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII, (13 June 2003); doi: 10.1117/12.480277
Show Author Affiliations
Irina G. Meerovich, A.N. Bach Institute of Biochemistry (Russia)
Victoria V. Jerdeva, A.N. Bach Institute of Biochemistry (Russia)
Gennadii A. Meerovich, A.N. Prokhorov General Physics Institute (Russia)
Valentina M. Derkacheva, State Scientific Ctr. NIOPIK (Russia)
Alexander P. Savitsky, A.N. Bach Institute of Biochemistry (Russia)


Published in SPIE Proceedings Vol. 4952:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII
David Kessel, Editor(s)

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