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Proceedings Paper

Photothermal microscopy of nano-objects
Author(s): Vladimir P. Zharov; Dmitri Lapotko
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Paper Abstract

The further development of a time-resolved photothermal (PT) method for sensing nano-scale absorbing targets is presented. This method is based on irradiation of nano-targets with a short laser pulse and time-resolved PT visualization of laser-induced thermal effects around targets. It is accomplished with a second probe beam that senses the target with an adjustable time delay after the pump laser pulse. The practical capability of this new approach is demonstrated for visualization of nano-scale gold particles (2-250 nm), liposomes (30-90 nm), Neutral Red (NR) particles (30-500 nm), and polystyrene beads as a calibration tool, with a tunable parametric pulse laser-OPO (420-570 nm, 0.10300 μJ, 8-ns width) as a pump laser and Raman-Shifter (639 nm, 13 ns width, 10 nJ) as a probe laser with a tunable delay of the probe pulse relative to thepump pulse in the range of 0-5000 ns. The further development of our PT technique is discussed with a focus on visualization of nano-scale absorbing zones during laser-cell interaction.

Paper Details

Date Published: 1 July 2003
PDF: 13 pages
Proc. SPIE 4960, Biomedical Optoacoustics IV, (1 July 2003); doi: 10.1117/12.479834
Show Author Affiliations
Vladimir P. Zharov, Univ. of Arkansas for Medical Sciences (United States)
Dmitri Lapotko, Luikov Heat and Mass Transer Institute (Belarus)

Published in SPIE Proceedings Vol. 4960:
Biomedical Optoacoustics IV
Alexander A. Oraevsky, Editor(s)

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