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Proceedings Paper

The diagnosis of lymph microcirculation in experimental studies on rat mesentery in vivo
Author(s): Ekateryna I. Galanzha; Valery V. Tuchin; Vladimir P. Zharov; Anastasia V. Solovieva; Tatyana V. Stepanova; Gregory E. Brill
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Paper Abstract

The many biological and medical problems associated with microlymphatic functioning and its disturbances at different diseases, include primary and secondary lymphedema, inflammation, lymphatic malformations, and so on. It is important both to establish an adequate animal model for study lymph microcirculation in vivo and to match it with corresponding diagnostic techniques. The rat mesentery has been successfully used in experiments focusing on the microcirculation, including small lymphatics. Among optical methods the transmittance microscopy is most widely employed to study microcirculation. We have undertaken following investigations: development and evaluation of capability of transmission microscopy for in vivo studies of microcirculation; obtaining of single cell images; estimation of lymph microcirculation parameters, including the relation of forward to backward flow in intact lymphatics; regulation of microlymphatic function by nitric oxide and study of microlymphatic disturbances at the experimental lymphedema. Although interesting data has been obtained, the transmission microscopy has the relatively low absorption sensitivity and prevents obtaining good absorption contrast. To obtain more comprehensive physiological data, the further development and improvement imaging of rat mesentery is discussed with focus on new combined optical imaging systems which integrate recent advances in video-transmission and photothermal (PT) microscopy, PT fluid velocimetry, and laser spectroscopy.

Paper Details

Date Published: 23 July 2003
PDF: 11 pages
Proc. SPIE 4965, Optical Diagnostics and Sensing in Biomedicine III, (23 July 2003); doi: 10.1117/12.479257
Show Author Affiliations
Ekateryna I. Galanzha, Saratov State Univ. (Russia)
Saratov State Medical Univ. (Russia)
Valery V. Tuchin, Saratov State Univ. (Russia)
Vladimir P. Zharov, Univ. of Arkansas for Medical Sciences (United States)
Anastasia V. Solovieva, Saratov State Univ. (Russia)
Tatyana V. Stepanova, Saratov State Medical Univ. (Russia)
Gregory E. Brill, Saratov State Medical Univ. (Russia)

Published in SPIE Proceedings Vol. 4965:
Optical Diagnostics and Sensing in Biomedicine III
Alexander V. Priezzhev; Gerard L. Cote, Editor(s)

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