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Proceedings Paper

In situ identification of bacteria size by light scattering
Author(s): Alvin Katz; Alexandra Alimova; Min Xu; Elizabeth Rudolph; Howard E. Savage; Mahendra Shah; Steven A. McCormick; Richard B. Rosen; Robert R. Alfano
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Paper Abstract

Optical transmission and light scattering measurements were performed on three species of bacteria with different sizes and shapes (Pseudomonas aeruginosa. Staphylococcus aureus and Bacillus subtilis). The average bacteria size was determined from transmission measurements by using the Gaussian Ray Approximation of Anomalous Diffraction Theory. A rescaled spectra combining multiple angular data was analyzed in the framework of the Rayleigh-Gans theory of light scattering in order to determine particle shape and size distribution. Particle size and shape as determined by both methods are in good agreement with size and shape measured by scanning electron microscopy. These results demonstrate that light scattering may be able to detect and identify microbial contamination in the environment.

Paper Details

Date Published: 23 July 2003
PDF: 4 pages
Proc. SPIE 4965, Optical Diagnostics and Sensing in Biomedicine III, (23 July 2003); doi: 10.1117/12.479188
Show Author Affiliations
Alvin Katz, Institute for Ultrafast Spectroscopy and Lasers, CUNY City College (United States)
Alexandra Alimova, Institute for Ultrafast Spectroscopy and Lasers, CUNY City College (United States)
Min Xu, Institute for Ultrafast Spectroscopy and Lasers, CUNY City College (United States)
Elizabeth Rudolph, Institute for Ultrafast Spectroscopy and Lasers, CUNY City College (United States)
Howard E. Savage, New York Eye and Ear Infirmary (United States)
Mahendra Shah, New York Eye and Ear Infirmary (United States)
Steven A. McCormick, New York Eye and Ear Infirmary (United States)
Richard B. Rosen, New York Eye and Ear Infirmary (United States)
Robert R. Alfano, Institute for Ultrafast Spectroscopy and Lasers, CUNY City College (United States)


Published in SPIE Proceedings Vol. 4965:
Optical Diagnostics and Sensing in Biomedicine III
Alexander V. Priezzhev; Gerard L. Cote, Editor(s)

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