Share Email Print
cover

Proceedings Paper

Confocal fluorescence techniques in industrial application
Author(s): Christian Eggeling; Karsten Gall; Kaupo Palo; Peet Kask; Leif Brand
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

The FCS+plus family of evaluation tools for confocal fluorescence spectroscopy, which was developed during recent years, offers a comprehensive view to a series of fluorescence properties. Originating in fluorescence correlation spectroscopy (FCS) and using similar experimental equipment, a system of signal processing methods such as fluorescence intensity distribution analysis (FIDA) was created to analyze in detail the fluctuation behavior of fluorescent particles within a small area of detection. Giving simultaneous access to molecular parameters like concentration, translational and rotational diffusion, molecular brightness, and multicolor coincidence, this portfolio was enhanced by more traditional techniques of fluorescence lifetime as well as time-resolved anisotropy determination. The cornerstones of the FCS+plus methodology will be shortly described. The inhibition of a phosphatase enzyme activity gives a comprehensive industrial application that demonstrates FCS+plus' versatility and its potential for pharmaceutical drug discovery.

Paper Details

Date Published: 19 June 2003
PDF: 9 pages
Proc. SPIE 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues, (19 June 2003); doi: 10.1117/12.478915
Show Author Affiliations
Christian Eggeling, Evotec Technologies GmbH (Germany)
Karsten Gall, Evotec OAI AG (Germany)
Kaupo Palo, Evotec Technologies GmbH (Germany)
Peet Kask, Evotec Technologies GmbH (Germany)
Institute of Experimental Biology (Estonia)
Leif Brand, Evotec Technologies GmbH (Germany)


Published in SPIE Proceedings Vol. 4962:
Manipulation and Analysis of Biomolecules, Cells, and Tissues
Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

© SPIE. Terms of Use
Back to Top