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Proceedings Paper

Results of UV laser application on biological material
Author(s): P. Alifano; Vincenzo Nassisi; Pier Paolo Pompa; A. Candido
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Paper Abstract

In this paper we report on the biological effects of XeCL laser irradiation on Staphylococcus epidermidis and Escherichia coli. UV interaction with cellular systems is responsible for photochemical, photothermal or photodecomposition processes. When short-wavelength UV radiation strikes biological material, the DNA is damaged causing cell killing, mutagenesis or carcinogenesis. We report on different effects of XeCl laser irradiation on two microbial systems; collection strain of Staphylococcus epidermidis (in suspension) and collection strains of Eschericha coli proficient or deficient in DNA recombination/repair pathways (irradiated on solid surfaces). In S epidermidis the 308 nm radiation can significantly enhanced the proliferation rates. In wild type E. coli cells the radiation did not stimulate the growth rates. Surprisingly, the 308 nm radiation elicited a very strong lethal effect on DNA recombination/repair-defective strains (harbouring the recA56 null mutation), even more pronounced than irradiation with a UV 254 nm germicidal lamp. The unknown mechanism responsible for this biological response is currently under investigation.

Paper Details

Date Published: 9 August 2002
PDF: 9 pages
Proc. SPIE 4762, ALT'01 International Conference on Advanced Laser Technologies, (9 August 2002); doi: 10.1117/12.478656
Show Author Affiliations
P. Alifano, Univ. degli Studi di Lecce (Italy)
Vincenzo Nassisi, Univ. degli Studi di Lecce (Italy)
Pier Paolo Pompa, Univ. degli Studi di Lecce (Italy)
A. Candido, Univ. degli Studi di Lecce (Italy)

Published in SPIE Proceedings Vol. 4762:
ALT'01 International Conference on Advanced Laser Technologies
Dan C. Dumitras; Maria Dinescu; Vitali I. Konov, Editor(s)

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