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Proceedings Paper

Some potential uses of photothermal detection for DNA microarray quantification
Author(s): Stephane Getin; Patrick Chaton; Francoise Vinet; Ludovic Poupinet; Catherine Pelle; Pierre Puget; Frederic Ginot; Armelle Novelli
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Paper Abstract

Photothermal deflection technique is a very sensitive mean to measure optical absorbance. This study is aimed at evaluating its potentiality in order to detect hybridation and to monitor quality control. The principle of the technique relies on the fact that nucleic acids present the property to absorb light between 220 nm to 280 nm. A first theoretical approach based on solving light and thermal equations demonstrates the ability of photothermal deflection to detect hybridisation. This point is checked through several experimentations with oligonucleotid with 32 mers targets lengths. An important point to stres is the specificity of the signals obtained and the ability of automation of the reading with image processing algorithms. At last we focus our attention on the ability of this technique to in-situ synthesis process. Our experimental study shows the ability of this characterisation with a detection sensitivity of one base.

Paper Details

Date Published: 18 July 2003
PDF: 6 pages
Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); doi: 10.1117/12.478353
Show Author Affiliations
Stephane Getin, CEA-LETI (France)
Patrick Chaton, CEA-LETI (France)
Francoise Vinet, CEA-LETI (France)
Ludovic Poupinet, CEA-LETI (France)
Catherine Pelle, CEA-LETI (France)
Pierre Puget, CEA-LETI (France)
Frederic Ginot, BioMerieux S.A. (France)
Armelle Novelli, BioMerieux S.A. (France)

Published in SPIE Proceedings Vol. 4966:
Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis
Dan V. Nicolau; Ramesh Raghavachari, Editor(s)

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