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Proceedings Paper

Mean cell size and collagen orientation from 2D Fourier analysis of confocal laser scanning microscopy and two-photon fluorescence microscopy on human skin in vivo
Author(s): Gerald W. Lucassen; Bernard L.G. Bakker; Sieglinde Neerken; Rob F. M. Hendriks
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Paper Abstract

We present results from 2D Fourier analysis on 3D stacks of images obtained by confocal laser scanning reflectance microscopy (CLSM) and two-photon fluorescence microscopy (2PM) on human skin in vivo. CLSM images were obtained with a modified commercial system (Vivascope1000, Lucid Inc, excitation wavelength 830 nm) equipped with a piezo-focusing element (350 μm range) for depth positioning of the objective lens. 2PM was performed with a specially designed set-up with excitation wavelength 730 nm. Mean cell size in the epidermal layer and structural orientation in the dermal layer have been determined as a function of depth by 2D Fourier analysis. Fourier analysis on microscopic images enables automatic non-invasive quantitative structural analysis (mean cell size and orientation) of living human skin.

Paper Details

Date Published: 9 July 2003
PDF: 9 pages
Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); doi: 10.1117/12.478335
Show Author Affiliations
Gerald W. Lucassen, Philips Research (Netherlands)
Bernard L.G. Bakker, Philips Research (Netherlands)
Sieglinde Neerken, Philips Research (Netherlands)
Rob F. M. Hendriks, Philips Research (Netherlands)


Published in SPIE Proceedings Vol. 4964:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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