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Proceedings Paper

Estimation of optical fluorescent impulse response kernel in combined time and wavelength spaces
Author(s): Olga V. Ivanova; Laura Marcu; Michael C. K. Khoo
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Paper Abstract

Current state of the knowledge in the field of optical diagnostics indicates that application of both steady state and time-resolved fluorescence spectroscopy permits the discrimination among normal and pathological tissues. Most data analysis algorithms, however, separately address either the spectral profile of the fluorescence emission or the time domain features of fluorescence decay of tissue sample. We present a novel system identification technique that allows for intrinsic Fluorescent Impulse Response Kernel (FIRK) estimation in combined time and wavelength spaces. The fluorescence experiment is described as a discrete two-dimensional single input - single output system. Modeling of FIRK is based on the characteristics of spectro-temporal fluorescence waveforms. Taking advantage of the exponentially decaying temporal behavior of the fluorescent output, we use a linear combination of Laguerre functions to model the fluorescence response as a function of time. To address the large variability of spectral profiles from different biological samples, we use the discrete Fourier series expansion to model the variation of fluorescence intensity across wavelength. The validation of the proposed approach is performed on simulated fluorescence data and fluorescence data measured from fluorescence lifetime standards. The algorithm is applicable for an effective recovery of intrinsic FIRK from any excitation light probe spectro-temporal waveforms. The retrieved FIRK can be further used for modeling and/or classification of pathological and physiological conditions of tissues.

Paper Details

Date Published: 29 July 2003
PDF: 12 pages
Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); doi: 10.1117/12.478213
Show Author Affiliations
Olga V. Ivanova, Univ. of Southern California (United States)
Cedars-Sinai Medical Ctr. (United States)
Laura Marcu, Univ. of Southern California (United States)
Cedars-Sinai Medical Ctr. (United States)
Michael C. K. Khoo, Univ. of Southern California (United States)

Published in SPIE Proceedings Vol. 4955:
Optical Tomography and Spectroscopy of Tissue V
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva M. Sevick-Muraca, Editor(s)

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