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Proceedings Paper

Detection and imaging of the reconstituted pyropheophorbide-cholesterol oleate labeled low-density lipoprotein in the HepG2 tumor
Author(s): Dana M. Blessington; Zhihong Zhang; Hui Li; Min Zhang; Lanlan Zhou; Jerry D. Glickson; Gang Zheng; Britton Chance
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Paper Abstract

We utilized the nude mouse model bearing the human hepatoblastoma G2 (HepG2) tumor and B-16 Murine Melanoma tumor to study the delivery and detection of the reconstituted Pyropheophorbide Cholesterol Oleate (r-pyroCE) molecular beacon. The delivery vehicle, low-density lipoprotein (LDL), labeled with the porphyrin derivative, was employed in response of the overexpression of LDL receptors in the HepG2 tumor. The B-16 melanoma tumor was also observed in this study for its overexpression of the LDL receptors. The tumors were imaged using the 3D low temperature scanner to produce images throughout several sliced sections of each tumor. The fluorescence signal of the pyropheophorbide was detected at 720nm when excited at 670nm in the tumor tissue. The uniform distribution of the signal in the HepG2 tumor shows extravasation of the beacon from the blood vessels. The B-16 tumor did not exhibit strong fluorescent signals and successful delivery as the HepG2 tumor outside the blood vessels and into the tumor tissue.

Paper Details

Date Published: 29 July 2003
PDF: 8 pages
Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); doi: 10.1117/12.478190
Show Author Affiliations
Dana M. Blessington, Univ. of Pennsylvania (United States)
Zhihong Zhang, Univ. of Pennsylvania (United States)
Hui Li, Univ. of Pennsylvania (United States)
Min Zhang, Univ. of Pennsylvania (United States)
Lanlan Zhou, Univ. of Pennsylvania (United States)
Jerry D. Glickson, Univ. of Pennsylvania (United States)
Gang Zheng, Univ. of Pennsylvania (United States)
Britton Chance, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 4955:
Optical Tomography and Spectroscopy of Tissue V
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva M. Sevick-Muraca, Editor(s)

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