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Proceedings Paper

NIR imaging the delivery of cathepsin B probe to breast tumors
Author(s): Lanlan Zhou; Dana M. Blessington; Zhihong Zhang; Aristid E. Lindenmayer; Ching Hsuan Tung; Ralph Weissleder; Britton Chance
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Paper Abstract

Proteases are involved in the invasion and metastasis of tumor cells. Cathepsin B overexpression has been shown in some neoplastic tissues. This study assesses the expression of Cathepsin B in the human fibrosarcoma (HT1080) in the mouse model by near-infrared (NIR) imaging. The nude mice were intravenously injected "a stealth probe" - an activable Cathepsin B sensing near-infrared fluorescence (NIRF) probe (24 hours before sacrifice) and the dye Cy5.5 (30 seconds before sacrifice). The animals were freeze-trapped and NIR images were obtained by the low temperature NIR scanner at the following excitation-emission wavelength pairs: 366, 450nm (NADH), 436, 520nm (FAD), and 670, 695nm (Cathepsin B probe). After imaging, the samples were submitted for histopathological evaluation. The tumor redox ratio NADH/(NADH+FAD) increased significantly because of the hypoxic state of tumor tissue with respect to normal tissue. The Cathepsin B probe was uniformly distributed throughout the tumor. This study indicated the efficient usage of the Cathepsin B probe in the molecular imaging for the detection of the early stage tumors.

Paper Details

Date Published: 29 July 2003
PDF: 8 pages
Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); doi: 10.1117/12.478188
Show Author Affiliations
Lanlan Zhou, Univ. of Pennsylvania (United States)
Dana M. Blessington, Univ. of Pennsylvania (United States)
Zhihong Zhang, Univ. of Pennsylvania (United States)
Aristid E. Lindenmayer, AmeriPath Consulting Pathologists Labs. (United States)
Ching Hsuan Tung, Massachusetts General Hospital (United States)
Ralph Weissleder, Massachusetts General Hospital (United States)
Britton Chance, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 4955:
Optical Tomography and Spectroscopy of Tissue V
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva M. Sevick-Muraca, Editor(s)

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