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Proceedings Paper

Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells
Author(s): Herbert Schneckenburger; Martina Kretzschmar; Michael Wagner; Wolfgang S. L. Strauss; Reinhard Sailer
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Paper Abstract

Fluorescence spectra and lifetime images of cultivated CHO cells incubated with the membrane marker laurdan are reported. The plasma membrane and intracellular membranes were distinguished using illumination of either the cell surface (by an evanescent wave) or of the whole cell. Parameters of membrane dynamics, probed by the generalized prolarization and the effective fluorescence lifetime of laurdan, were correlated with temperature, age and growth phase of the cells.

Paper Details

Date Published: 19 June 2003
PDF: 7 pages
Proc. SPIE 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues, (19 June 2003); doi: 10.1117/12.478118
Show Author Affiliations
Herbert Schneckenburger, Fachhochschule Aalen (Germany)
Institut fur Lasertechnologien in der Medizin und Messtechnik/Univ. Ulm (Germany)
Martina Kretzschmar, Fachhochschule Aalen (Germany)
Michael Wagner, Fachhochschule Aalen (Germany)
Wolfgang S. L. Strauss, Institut fur Lasertechnologien in der Medizin und Messtechnik/Univ. Ulm (Germany)
Reinhard Sailer, Institut für Lasertechnologien in der Medizin und Meßtechnik/Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 4962:
Manipulation and Analysis of Biomolecules, Cells, and Tissues
Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

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