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Proceedings Paper

Gene expression analysis of perennial ryegrass (Lolium perenne) using cDNA microarrays
Author(s): Eng-Kok Ong; Tim Sawbridge; Tracie Webster; Michael Emmerling; Nga Nguyen; Katrina Nunan; Matthew O'Neill; Fiona O'Toole; Carolyn Rhodes; Jason Simmonds; Pei Tian; Katherine Wearne; Amanda Winkworth; German Spangenberg
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Paper Abstract

Perennial ryegrass (Lolium perenne) is a major forage grass of temperate pastures. A genomics program has been undertaken generating over 52,000 expressed sequence tags (ESTs). Cluster analysis of the ESTs identified approximately 14,600 ryegrass unigenes. In this report, we described the application of ryegrass unigene cDNAs to produce ryegrass 15K microarray. Fifteen microarray hybridisations were performed with labeled total RNA isolated from a variety of plant organs and developmental stages. In a proof of concept, gene expression profiling of ryegrass ESTs using the 15K unigene microarrays has been established using several known genes and two cluster analysis approaches (parallel coordinate planes plot and hierarchical clustering). The expression profile of the known genes (e.g. rubisco and invertase) corresponds well with published data. The microarray expression profile of a ryegrass putative root specific kinase gene was also verified with Northern blotting. This combination of DNA microarray hybridisations and cluster analysis can be applied as a tool for the identification of novel sequences of unknown function.

Paper Details

Date Published: 18 July 2003
PDF: 11 pages
Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); doi: 10.1117/12.478075
Show Author Affiliations
Eng-Kok Ong, La Trobe Univ. (Australia)
Tim Sawbridge, La Trobe Univ. (Australia)
Tracie Webster, La Trobe Univ. (Australia)
Michael Emmerling, La Trobe Univ. (Australia)
Nga Nguyen, La Trobe Univ. (Australia)
Katrina Nunan, La Trobe Univ. (Australia)
Matthew O'Neill, La Trobe Univ. (Australia)
Fiona O'Toole, La Trobe Univ. (Australia)
Carolyn Rhodes, La Trobe Univ. (Australia)
Jason Simmonds, La Trobe Univ. (Australia)
Pei Tian, La Trobe Univ. (Australia)
Katherine Wearne, La Trobe Univ. (Australia)
Amanda Winkworth, La Trobe Univ. (Australia)
German Spangenberg, La Trobe Univ. (Australia)

Published in SPIE Proceedings Vol. 4966:
Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis
Dan V. Nicolau; Ramesh Raghavachari, Editor(s)

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