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Proceedings Paper

New implementation of second harmonic generation microscopy for three-dimensional resolution
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Paper Abstract

We present a fast scanning transmission-mode confocal scanning laser microscope system based on the use of a second harmonic generation (SHG) crystal for signal detection. The quadratic intensity dependence of SHG is exploited to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal plays the role of a virtual pinhole that remains self-aligned without a need for de-scanning. We demonstrate that this new microscope method produces images with higher contrast and less speckle than transmission scanning microscopy with linear detection.

Paper Details

Date Published: 10 July 2003
PDF: 8 pages
Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478032
Show Author Affiliations
Changhuei Yang, Ecole Superieure de Physique et de Chimie Industrielles, INSERM-CNRS (France)
Jerome Mertz, Ecole Superieure de Physique et de Chimie Industrielles, INSERM-CNRS (France)


Published in SPIE Proceedings Vol. 4963:
Multiphoton Microscopy in the Biomedical Sciences III
Ammasi Periasamy; Peter T. C. So, Editor(s)

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