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Proceedings Paper

Photo-induced flip-flop of amphiphilic membrane markers
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Paper Abstract

We demonstrate localized photo-induced flip-flop of stilbazolium markers in model lipid bilayer membranes. The flip-flop mechanism and dynamics are determined by combined two-photon excited fluorescence and second harmonic generation microscopy. Upon illumination of labeled membranes with a femtosecond laser beam, two-photon absorption induced photo-isomerization provokes a significant increase in the cis-marker population whose flip-flop rate was determined to be at least a thousand times faster than that for trans-markers.

Paper Details

Date Published: 10 July 2003
PDF: 4 pages
Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478022
Show Author Affiliations
Thomas Pons, Ecole Superieure de Physique et de Chimie Industrielles, INSERM-CNRS (France)
Jerome Mertz, Ecole Superieure de Physique et de Chimie Industrielles, INSERM-CNRS (France)


Published in SPIE Proceedings Vol. 4963:
Multiphoton Microscopy in the Biomedical Sciences III
Ammasi Periasamy; Peter T. C. So, Editor(s)

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