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Proceedings Paper

Automatic segmentation of histological structures in normal and neoplastic mammary gland tissue sections
Author(s): Rodrigo Fernandez-Gonzalez; Thomas Deschamps; Adam Idica; Ravikanth Malladi; Carlos Ortiz de Solorzano
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Paper Abstract

In this paper we present a scheme for real time segmentation of histological structures in microscopic images of normal and neoplastic mammary gland sections. Paraffin embedded or frozen tissue blocks are sliced, and sections are stained with hematoxylin and eosin (H&E). The sections are then imaged using conventional bright field microscopy. The background of the images is corrected by arithmetic manipulation using a "phantom." Then we use the fast marching method with a speed function that depends on the brightness gradient of the image to obtain a preliminary approximation to the boundaries of the structures of interest within a region of interest (ROI) of the entire section manually selected by the user. We use the result of the fast marching method as the initial condition for the level set motion equation. We run this last method for a few steps and obtain the final result of the segmentation. These results can be connected from section to section to build a three-dimensional reconstruction of the entire tissue block that we are studying.

Paper Details

Date Published: 9 July 2003
PDF: 12 pages
Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); doi: 10.1117/12.477996
Show Author Affiliations
Rodrigo Fernandez-Gonzalez, Lawrence Berkeley National Lab. (United States)
Univ. of California at Berkeley/Univ. of Calfornia at San Fran Joint Group Program in Bioengineering (United States)
Thomas Deschamps, Lawrence Berkeley National Lab. (United States)
Adam Idica, Lawrence Berkeley National Lab. (United States)
Ravikanth Malladi, Lawrence Berkeley National Lab. (United States)
Carlos Ortiz de Solorzano, Lawrence Berkeley National Lab. (United States)


Published in SPIE Proceedings Vol. 4964:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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