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Proceedings Paper

Imaging of cell layers by differential phase contrast optical coherence microscopy
Author(s): Christoph K. Hitzenberger; Markus Sticker; Michael Pircher; Erich Gotzinger; Harald Sattmann; Adolf Friedrich Fercher
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Paper Abstract

Optical coherence tomography (OCT) measures and images the backscattering potential of transparent and semi-transparent samples. Recently, we reported on an extension of OCT with a differential phase contrast (DPC) technique. We have improved the technique and developed a DPC optical coherence microscope (OCM) which allows to image sub-wavelength optical path differences occurring between a narrow beam probing a sample and its surrounding. This allows the visualization of small transversal refractive index variations close to a selected interface. We report on the method and present first images of a single cell layer. The cells act as phase objects, and by imaging the phase properties the contrast is improved as compared to intensity images.

Paper Details

Date Published: 8 July 2003
PDF: 4 pages
Proc. SPIE 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, (8 July 2003); doi: 10.1117/12.477890
Show Author Affiliations
Christoph K. Hitzenberger, Univ. of Vienna (Austria)
Markus Sticker, Univ. of Vienna (Austria)
Michael Pircher, Univ. of Vienna (Austria)
Erich Gotzinger, Univ. of Vienna (Austria)
Harald Sattmann, Univ. of Vienna (Austria)
Adolf Friedrich Fercher, Univ. of Vienna (Austria)

Published in SPIE Proceedings Vol. 4956:
Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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