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Proceedings Paper

Molecular species-sensitive optical coherence tomography using coherent anti-stokes Raman scattering spectroscopy
Author(s): Daniel L. Marks; Jeremy Bredfeldt; Selezion Hambir; Dana D. Dlott; Barbara Kitchell; Martin Gruebele; Stephen A. Boppart
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Paper Abstract

We present our progress in developing a novel technique and instrument that images specific molecular species in biological tissues using Optical Coherence Tomography (OCT). Standard OCT instruments measure only the scattering from structural features, such as refractive index changes. We utilize Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy, a nonlinear optics technique that can selectively stimulate molecular groups, to gather compositional information from the sample. Being a coherent process, our instrument will produce interference between the nonlinear anti-Stokes signal produced in the sample and a reference molecular sample to both exclude background and nonresonant signals and range features in the tissue. Because of this, we will also gain the benefits of sensitivity that interferometry can provide. By utilizing the tunability of an optical parametric oscillator, we can address a range of molecular resonances from 1500 cm-1 to 3500 cm-1. This frequency range offers the possibility of measuring the distributions and densities of proteins, lipids, and nuclear material that we believe will be useful for determining the early presence of epithelial carcinomas. We demonstrate the principle of this imaging method by producing interference between two separately produced CARS signals from the same probe and Stokes beams.

Paper Details

Date Published: 8 July 2003
PDF: 5 pages
Proc. SPIE 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, (8 July 2003); doi: 10.1117/12.477787
Show Author Affiliations
Daniel L. Marks, Univ. of Illinois/Urbana-Champaign (United States)
Beckman Institute for Advanced Science and Technology (United States)
Jeremy Bredfeldt, Univ. of Illinois/Urbana-Champaign (United States)
Beckman Institute for Advanced Science and Technology (United States)
Selezion Hambir, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois/Urbana-Champaign (United States)
Dana D. Dlott, Univ. of Illinois/Urbana-Champaign (United States)
Barbara Kitchell, Univ. of Illinois/Urbana-Champaign (United States)
Martin Gruebele, Univ. of Illinois/Urbana-Champaign (United States)
Stephen A. Boppart, Univ. of Illinois/Urbana-Champaign (United States)
Beckman Institute for Advanced Science and Technology (United States)


Published in SPIE Proceedings Vol. 4956:
Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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