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Proceedings Paper

Modeling and simulation of integrated luminescence detection platforms
Author(s): Khaled Salama; Helmy Eltoukhy; Arjang Hassibi; Abbas El Gamal
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Paper Abstract

We developed a simulation model of an integrated CMOS-based imaging platform for use with bioluminescent DNA microarrays. We formulate the complete kinetic model of ATP based assays and luciferase label-based assays. The model first calculates the number of photons generated per unit time, i.e., photon flux, based upon the kinetics of the light generation process of luminescence probes. The photon flux coupled with the system geometry is then used to calculate the number of photons incident on the photodetector plane. Subsequently the characteristics of the imaging array including the photodetector spectral response, its dark current density, and the sensor conversion gain are incorporated. The model also takes into account different noise sources including shot noise, reset noise, readout noise and fixed pattern noise. Finally, signal processing algorithms are applied to the image to enhance detection reliability and hence increase the overall system throughput. We will present simulations and preliminary experimental results.

Paper Details

Date Published: 18 July 2003
PDF: 11 pages
Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); doi: 10.1117/12.477781
Show Author Affiliations
Khaled Salama, Stanford Univ. (United States)
Helmy Eltoukhy, Stanford Univ. (United States)
Arjang Hassibi, Stanford Univ. (United States)
Abbas El Gamal, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 4966:
Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis
Dan V. Nicolau; Ramesh Raghavachari, Editor(s)

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