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Proceedings Paper

Melanin and the cellular effects of ultrashort-pulse near-infrared laser radiation
Author(s): Randolph D. Glickman; Neeru Kumar; Benjamin A. Rockwell; Gary D. Noojin; Michael L. Denton; David J. Stolarski
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Paper Abstract

Our research into laser bioeffects has increasingly focused on cytotoxic mechanisms affecting genomic expression and programmed cellular stress responses. In the context of DNA damage, we previously reported that more DNA strand breaks were produced in cultured retinal pigment epithelium (RPE) cells exposed to ultrashort pulse, than to CW, near-infrared (NIR) laser radiation. To test the hypothesis that RPE melanin was the cellular chromophore responsible for mediating this damage, the experiments were repeated with a line of human-derived RPE cells that could be grown in culture expressing varying levels of pigmentation. Lightly-pigmented cells were either unexposed, or exposed to the output of a Ti:Sapphire laser producing 810 nm light in mode-locked pulses (48-fsec at 80 MHz), or as CW radiation. Cells were irradiated at 160 W/cm2 or 80 W/cm2 (the estimated ED50 or half-ED50 for a retinal lesion). Immediately following the laser exposure, cells were processed for the comet assay. Longer "comet" tails and larger "comet" areas indicated more DNA strand breaks. In lightly-pigmented RPE cells, the overall comet assay differences among the laser-exposed groups were smaller than those observed in our earlier experiments which utilized highly pigmented primary cells. The comet tail lengths of cells exposed to the mode-locked pulses at the ED50, however, were significantly longer than those of the controls or the CW-exposed cells. The other comet assay parameters examined (tail moment, comet area) did not show consistent differences among the groups. While these results support the involvement of melanin in the ultrashort pulse laser-induced damage to DNA, they do not exclude the involvement of other cellular chromophores. Some preliminary experiments describing other measures of cellular stress responses to laser-induced oxidative stress are described.

Paper Details

Date Published: 27 August 2003
PDF: 9 pages
Proc. SPIE 4961, Laser-Tissue Interaction XIV, (27 August 2003); doi: 10.1117/12.477653
Show Author Affiliations
Randolph D. Glickman, Univ. of Texas Health Science Ctr. (United States)
Neeru Kumar, Univ. of Texas Health Science Ctr. (United States)
Benjamin A. Rockwell, Air Force Research Lab. (United States)
Gary D. Noojin, Northrop Grumman IT (United States)
Michael L. Denton, Northrop Grumman IT (United States)
David J. Stolarski, Northrop Grumman IT (United States)

Published in SPIE Proceedings Vol. 4961:
Laser-Tissue Interaction XIV
Donald Dean Duncan; Sean J. Kirkpatrick; Andres Kriete; Steven L. Jacques, Editor(s)

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