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Proceedings Paper

Exogenous specific fluorescence marker location reconstruction using surface fluorescence imaging
Author(s): Garashi Avital; Israel Gannot; Victor V. Chernomordik; Gallya Gannot; Amir H. Gandjbakhche
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Paper Abstract

Diseased tissue may be specifically marked by an exogenous fluorescent marker and then, following laser activation of the marker, optically and non-invasively detected through fluorescence imaging. Interaction of a fluorophore, conjugated to an appropriate antibody, with the antigen expressed by the diseased tissue, can indicate the presence of a specific disease. Using an optical detection system and a reconstruction algorithm, we were able to determine the fluorophore’s position in the tissue. We present 3D reconstructions of the location of a fluorescent marker, FITC, in the tongues of mice. One group of BALB/c mice was injected with squamous cell carcinoma (SqCC) cell line to the tongue, while another group served as the control. After tumor development, the mice’s tongues were injected with FITC conjugated to anti-CD3 and anti-CD 19 antibodies. An Argon laser excited the marker at 488 nm while a high precision fluorescent camera collected the emitted fluorescence. Measurements were performed with the fluorescent marker embedded at various simulated depths. The simulation was performed using agarose-based gel slabs applied to the tongue as tissue-like phantoms. A biopsy was taken from every mouse after the procedure and the excised tissue was histologically evaluated. We reconstruct the fluorescent marker’s location in 3D using an algorithm based on the random walk theory.

Paper Details

Date Published: 29 July 2003
PDF: 8 pages
Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); doi: 10.1117/12.476803
Show Author Affiliations
Garashi Avital, Tel-Aviv Univ. (Israel)
Israel Gannot, Tel-Aviv Univ. (Israel)
National Institute of Child Health and Development (United States)
Victor V. Chernomordik, National Institutes of Health (United States)
Gallya Gannot, Tel-Aviv Univ. (Israel)
Amir H. Gandjbakhche, National Institutes of Health (United States)

Published in SPIE Proceedings Vol. 4955:
Optical Tomography and Spectroscopy of Tissue V
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva M. Sevick-Muraca, Editor(s)

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