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Proceedings Paper

Antimicrobial effect of Er:YAG laser irradiation in infected root canals: an in vitro study
Author(s): Marcelo Sampaio Moura; Fatima A. A. Zanin; Aldo Brugnera; Cyntia Rodrigues de Araujo Estrela; Carlos Estrela; Jesus Djalma Pecora
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Paper Abstract

This study evaluated in vitro the antimicrobial action of Er:YAG laser in infected root canals. A total of 36 human anterior teeth were prepared, sterilized and inoculated with suspensions of S. aureus, E. faecalis, P aeruginosa, B. subtilis and C. albicans. After the contamination period (28 days), the teeth were irrigated with sterile distilled water or 1% sodium hypochlorite and, then, irradiated with an Er:YAG laser (wavelength 2094 nm) with two different evaluation parameters: 52 mJ and 110 mJ laser output at the fiber tip. After the irradiation, the teeth were irrigated with sterile distilled water and the solutions were collected with sterile paper points and incubated in Letheen Broth for 48 hours. After this period 0,1 ml of this solution was transferred to 10 ml of Brain Heart Infusion and incubated for 48 hours. After 72 hours a new collect ion was done and incubated again. Based on the data, it was concluded that the distilled water or 1% sodium hypochlorite irradiated or not with an Er:YAG laser with two different parameters: 10 Hz, 110 mJ input and 52 mJ output, and 10 Hz, 250 mJ input and 110 mJ output had no antimicrobial activity in the root canals contaminated with the following microorganisms: Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans.

Paper Details

Date Published: 3 July 2003
PDF: 8 pages
Proc. SPIE 4950, Lasers in Dentistry IX, (3 July 2003); doi: 10.1117/12.476535
Show Author Affiliations
Marcelo Sampaio Moura, Univ. de Sao Paulo (Brazil)
Fatima A. A. Zanin, Univ. Federal do Rio de Janeiro (Brazil)
Aldo Brugnera, Univ. Federal do Rio de Janeiro (Brazil)
Cyntia Rodrigues de Araujo Estrela, Univ. Federal de Goias (Brazil)
Carlos Estrela, Univ. de Sao Paulo (Brazil)
Jesus Djalma Pecora, Univ. de Sao Paulo (Brazil)

Published in SPIE Proceedings Vol. 4950:
Lasers in Dentistry IX
Peter Rechmann; Daniel Fried; Thomas Hennig, Editor(s)

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