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Proceedings Paper

Polarized light (λ400-2000 nm): a description of the wound healing process using immunohistochemical analysis
Author(s): Bruno Pereira Campanha; Antonio Luiz Barbosa Pinheiro; Ruben Weismann; Marilia Gerhardt de Oliveira; Luciana Maria Pedreira Ramalho
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Paper Abstract

Wound healing is a complex process that involves several metabolic and cellular responses. Several methods of improving wound healing have been suggested. Polarized light was suggested as a new method of biostimulation, improving the quality of the healing process. The aim of this work was to investigate the effects of polarized light (λ400 - λ2000nm) on the healing of cutaneous wounds using immunohistochemical analysis. Eighteen healthy male young adults Wistar rats were used. Standardized wounds were created on the dorsum of the rat. The wounds were illuminated with the polarized light source at doses of 20 or 40J/cm2. Non-illuminated animals acted as controls. The first application of the light was performed immediately after the surgical procedure and repeated at every 48 hours during seven days. Twenty-four hours after the last irradiation the animals were humanely sacrificed. The slices were immunomarked by α SMA and by Picrosírius stain. The results of the histological analysis shows that the healing process on illuminated subjects was characterized by increased amount of collagen fibers when compared to control wounds. On the other hand, expressive amounts of myofibroblasts were also observed on illuminated specimens, when compared to non-irradiated animals, especially with a dose of 20J/cm2. It is concluded that the use of polarized light had influence on the proliferation of myofibroblasts as well as on the amount of collagen fibers, being this best observed when small doses are used.

Paper Details

Date Published: 3 July 2003
PDF: 6 pages
Proc. SPIE 4950, Lasers in Dentistry IX, (3 July 2003); doi: 10.1117/12.476430
Show Author Affiliations
Bruno Pereira Campanha, Pontificia Univ. Catolica do Rio Grande do Sul (Brazil)
Antonio Luiz Barbosa Pinheiro, Univ. Federal da Bahia (Brazil)
Univ. do Vale do Paraíba (Brazil)
Ruben Weismann, Pontificia Univ. Catolica do Rio Grande do Sul (Brazil)
Marilia Gerhardt de Oliveira, Pontificia Univ. Catolica do Rio Grande do Sul (Brazil)
Luciana Maria Pedreira Ramalho, Univ. Federal da Bahia (Brazil)


Published in SPIE Proceedings Vol. 4950:
Lasers in Dentistry IX
Peter Rechmann; Daniel Fried; Thomas Hennig, Editor(s)

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